Labsystems Luminoskan RT Microplate Reader. 0% and ± 0. 2. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. Setup recommendations for SpectraMax. TGFα release was calculated as described in the Results. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). #N207) LAL Reagent Water (Lonza cat. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. , Sunnyvale, CA, USA) at 595 nm. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. Molecular Devices SpectraMax Gemini XS Microplate Reader. Number of flashes. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). The membranes were. SPECTRAmax® 190. gp70 blocking. application or budget. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. SpectraMax 190 reads 96-well and strip-well plates. SpectraMax Plus 384 measuring instruments pdf manual download. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. 10. , Sunnyvale, CA). 3. , Fremont, CA, USA). SpectraMax Multi-Mode Microplate Readers. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. Software (SoftMax Pro ® software or comparable plate reader software). Operators can conveniently select up to 6 wavelengths at a time for. Analysis of ALA by Ehrlich’s Reagent Assay. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . The absorbance was measured at 340 nm for 2 min at a 15 s time interval. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. 7. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. SpectraMAX 340 plate reader (Molecular Devices). When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. 5 mL sample or standard solution was well mixed with 0. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. All strains were tested three times and the results averaged. #N207) LAL Reagent Water (Lonza cat. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). AoSMCs were seeded into 6-well culture plates. Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. The ratio of red to green fluorescence intensity was calculated. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. reader (Molecular Devi ces). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Alkaline phosphatase (ALP) activity. SpectraMax Multi-Mode Microplate Readers. These models can no longer be repaired. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 003 OD Stray light ≤ 0. 6) and 0. control or celatrol-treated cells. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). . The absorbance was measured at 340 nm for 2 min at a 15 s time interval. 0. P. 5. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). The concentration of TNFα in the experimental. 305 reference level saturation. SpectraMAX 340. California Lightworks uses only the highest quality LEDs from Osram. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. Spectral Range. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. Awareness Technology, Inc. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. Both readers feature an 8-channel read head for fast reads and. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. SpectraMax 384 Plus. edu Contact Phone: 216-368-8962. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Manager: Yinghua Chen, PhD Contact Email: yinghua. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. 2 were considered negative for IgM antibody. Emission. The sensitivities of the hormones are Prolactin: 0. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Molecular Devices SpectraMax M5e Microplate Reader. 25–0. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Percent viability was calculated by comparison with the control group. (1993). Stat Fax 4700 Microstrip Reader. SpectraMax Multi-Mode Microplate Readers. 312 gain calibration failed. Calculating % of total mediator release in RBL assay. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. . 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. 1% N-1-naphtylethylenediamine dihydrochloride, 2. used. The ABS reader ranges from. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Data were expressed as the mean percent viable cell vs. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). Immunofluorescence and Aggregation Analysis. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. Cell proliferation was measured using the MTT method. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. Isolation of RNA and RT-PCR. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). The SpectraMax® iD3 Multi Mode Microplate . 5 mL of 1 M acetate buffer (pH 4. ac. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Molecular Devices Spectramax 340 Plate Reader Repair. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Crowder, Ziqian Zeng, Alissa N. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. using SpectraMax i3 (Molecular devices, San Jose, USA). . Although all samples initially were analyzed at the same volume, the volume of sample. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. , San Jose, CA) using Softmax Pro 7 software. plates can be used in the SPECTRAmax PLUS 384. Amino Acid Analysis. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. , Sunnyvale, CA, USA) at a wavelength of 450 nm. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 5. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. 5. ATI/240, 250. Assay setup for a 384-well low-volume plate. Rate of fibrinolysis. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. Emission. Each experiment was performed in duplicate. TDP-43 stress granular cells were treated with 70% ice-cold. The values were corrected for nonspecific background by subtracting the OD 650. 3. Microplate Spectrophotometers. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. Description 1. SpectraMAX 340 microtiter plate reader. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. SpectraMAX 340. Awareness Technology Inc. Briefly, cells were seeded into a. 5. Microplate Readers. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Figure 1. #ABS PLUS) Methods. , Sunnyvale CA). 2. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. chen@case. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 30. Details. Samples were plated in duplicate (r=0. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. These robust readers have been placed in labs from Antarctica to the. The reaction mixture was incubated for 5 min. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices’ PathCheck® sensor technology. SPECTRAmax® 190. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. or microplate reader (e. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. Call 1-800-745-2710. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). 003 OD Stray light ≤ 0. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. The SPECTRAmax 340PC can measure. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. Wavelength Range. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. The contents of the. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. View the Molecular Devices website. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. The absorbances were being measured at 515 nm. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Subsequently, the mixture was heated at 100 °C for 10. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. Examples: “Press carefully to engage,” “Do not press down. Number of flashes. 2 M DEEMM were mixed in a microtube,. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. , Sunnyvale, CA). IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. more on but is otherwise untested and sold AS-IS. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. Binding of capture antibodies to polystyrene beads and biotinylation. 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 314 low lamp level warning. 7 macrophages were obtained from ATCC (Manassas, VA). Cell culture 3T3 fibroblasts and RAW 264. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. TGFα release was calculated as described in the Results. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). 5. Or scan up to 384 wells at any time to determine the best assay conditions. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Molecular Devices Corporation 1311. Microplate Spectrophotometer Operator’s Manual. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. TDP-43 stress granular cells were treated with 70% ice-cold. 5. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Money Saved: $340. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). 6. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Or scan up to 384 wells at any time to determine the best assay conditions. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Wavelength Selection. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. To measure the cell biomass (g/L),. Each experiment was performed in duplicate and repeated at. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. This assay measures the two major sugars in wine, glucose and fructose. 5% phosphoric acid) in microplates at room temperature for 10 min. The SPECTRAmax 340PC can measure. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The adapter is. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. 1 mL of acetylacetone. control. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. g. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. TGFα release was calculated as described in the Results. 97), and each plate contained at least six positive and six negative controls. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). using SpectraMax i3 (Molecular devices, San Jose, USA). The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. 30 µs. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Temperature Range: Ambient + 4 °C up to 45 °C. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 316 grating motor driver faulty. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. Details. Microplate Readers. . SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. Molecular Devices SpectraMAX 340 Microplate Reader. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Molecular Devices SPECTRAmax. Analysis of ALA by Ehrlich’s Reagent Assay. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). Microplate Spectrophotometer Operator’s Manual. Acceptor: 665/10 nm. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Inter-assay variation was prevented by testing all of the samples on the same day. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. 00. The Catalina. Our absorbance plate readers feature our PathCheck Sensor technology and. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. Donor: 616/10 nm. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 00 USD. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. plates can be used in the SPECTRAmax PLUS 384. A. Molecular Devices SpectraMax M5e Microplate Reader. Vessel Sprout Formation Assay. using SpectraMax i3 (Molecular devices, San Jose, USA). Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. Data were expressed as the mean LPS-treated cells (100%) vs. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. 3. Molecular Devices SpectraMax M5e Microplate Reader $26,500. Samples were analyzed in triplicates. SpectraMax® Microplate Readers and SoftMax® Pro Software. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Intra- and inter-assay coefficients of variation were lower than 8. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax QuickDrop Micro-Volume Spectrophotometer. Manager: Yinghua Chen, PhD Contact Email: yinghua. 0, MSD) or Spectramax 340 (Molecular Devices). 06 ng/mL; FSH: 0. Driessen*,MaaikeA. Labsystems Luminoskan RT Microplate Reader. 313 reference gain check fail. 00 USD.